منابع مشابه
Measurement of amylase activity.
The Nelson photoelectric method for the determination of glucose in biological fluids (1) has proved to be the method of choice in this laboratory and in many others, owing to its reproducibility, stability of color, and excellent agreement with the Beer-Lambert law. Nelson, however, states that the method is unsuitable for the determination of diastatic activity. Since it is convenient and eco...
متن کاملMeasurement of Amylase Activity*
The Nelson photoelectric method for the determination of glucose in biological fluids (1) has proved to be the method of choice in this laboratory and in many others, owing to its reproducibility, stability of color, and excellent agreement with the Beer-Lambert law. Nelson, however, states that the method is unsuitable for the determination of diastatic activity. Since it is convenient and eco...
متن کاملRecent advances in measurement of amylase activity--a comparative study.
We evaluated four kinetic amylase procedures with respect to kinetics, analytical range, blank rates, reagent stability, reagent impurities, interfering substances, and intrinsic sensitivities. Each of the methods is shown to have its own unique advantages and disadvantages. A preliminary discussion of some alternative methods, in which glycosidic p-nitrophenyl alpha-oligosaccharides are substr...
متن کاملKinetic nephelometric procedure for measurement of amylase activity in serum.
We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the n...
متن کاملAmylase inhibitory activity of some macrolichens in Mazandaran province, Iran
Introduction: α-amylase is a major form of amylase found in humans and other mammals. It is the special key enzyme involved in carbohydrates breakdown. Inhibition of this enzyme could be used in treatment of diabetes. In this study, the effect of ten Iranian macrolichens on alpha amylase were tested. Methods: Different concentrations of the extracts (25, 50 and 75 mg/ml) were incubated wi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1950
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56358-4